The concentration of total free thiols in the protein. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. Timothy Bolus, MPM, MBA, Compliance Program Manager. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. SpectraMax 250 User. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. BMG LABTECH. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The OptiMax by Molecular Devices is a tunable microplate reader. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. measurements in compatible Molecular Devices® microplate readers. g. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. adapter plate in drawer of Plate Reader with well A1 in position A1 of the drawer. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Reading Capabilities: cuvette or microplate. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 2 mm x 86 mm) Height: between 13. It measures the optical density (OD) of samples in both 96- and 384-well. Microplate is validated by molecular devices for the FLIPR reader. Compare this item. 10. 3. Click on the crosshair and move it so that it is centered over the well image. Insert the filled microplate into the drawer, matching well A1 with position A1. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Other technologies such as imaging. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). Reactions catalyzed by firefly and *Renilla luciferases. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. For more sample throughput, both readers can be easily integrated into full roboticThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Formats available are strip washers, full plate washers and combination washer-dispensers. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. tecHnical SPecificationS Performance Specifications Measurement range: 400–750 nm 0–4. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). Step 2: Launch SoftMax Pro Software. A microplate reader as . Keywords: fluorescence polarization, trf, fret, igg. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. SmartReader 96: Designed for optimal ELISA Testing. For more sample throughput, the system canRating: 3. SOP Title: Use and. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. flexibility of monochromator-based systems. 300 µL of Detergent Compatible Bradford reagent was added to each well. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Detergent Compatible Bradford assay. 96, 384, 24, 12, 6 well types. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. This page contains the. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. #N207) LAL Reagent Water (Lonza cat. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 96-well flat clear bottom white TC-treated microplates (Corning cat. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. These models can no longer be repaired. *Simple analysis and optimization. Fluorescence Microplate Reader Comparison Chart. in a 96-well microplate. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. A single-mode plate reader is a good starting point. SpectraDrop High-Throughput Screening (HTS) Kit. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 7. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. #G7294). of 0. Molecular Devices' microplate readers. . You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 48 W x 12. Monochromatic filter is equipped. The CellTiter-Glo assay from Promega uses luciferase. Microplate readers can be either single-function instruments or “multimode. 96-well-plate-scan-time-2-colors-1-site-well. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert. We would like to show you a description here but the site won’t allow us. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Step 3: Click “Settings” and a screen will appear (Figure 1). The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Also refer to this Multimode Reader Comparison Chart. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. San Jose, California, June 5, 2018. Temperature control. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. 96-well: 0. /well integration in 96-well luminescence mode and 0. 5 mm and 15. This . com. Reconstitution of. 40266. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. Molecular Devices' microplate readers. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. ***Figure 1. Our microplate. The color touch screen provides a visual user interface, making programming fast and intuitive. 63. As a result, assay flexibility is consequence-free. Specifications for the SpectraMax L microplate reader. 96-well microplate; Immunoassay analyzer;VersaMax price. BSA concentration (µg/mL) Average OD. Reconstitution of reagents. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. ± 0. (FP) configured microplate readers. white 96 well plate and 75. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. While they play an important role in generating data from a wide range of. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. Detergent Compatible Bradford assay. (96, 384, or 1536 wells) and the "Select Specific". Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Read the microplate Step 1: Place the microplate in the reader. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Model:. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. Detection by Low noise PMT with simultaneous digital and analog modes. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Microscopy. #W50-100). Products : SpectraMax M2/M2e reader, SpectraMax. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. #SpectraMax L Config) Methods. 3 OD at 200 nm. analysis software. 012 fmol 0. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. #0310-5227) 96-well cell wash head (Molecular Devices Cat. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Learn more. 2 OD at 200 nm. Key applications: ELISA, nucleic acid, endotoxin testing. The iMark. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. Ordering information FlexStation 3 Microplate Reader Part Number: FLEX3 • FlexStation 3 Base System Consumables 96-Well, FlexStation Pipet Tips (Black) Part Number: 9000-0911 • 200 µL capacity • Includes 3 vials each of FLIPR Calcium 6 and 6-QF dyes Reagents FLIPR® Calcium 6 Assay Evaluation Kit Part Number. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. Average StDev (mP) 1. Flexible Microplate Format The GEMINI system allows you to select from 6-, 12-, 24-, 48-, 96- and 384-well microplate formats for your fluorometric measurements. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Compare plate readersThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 48 W x 12. Learn more about how absorbance is measured, and some key applications that utilize. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. Fluorescent DNA quantitation. Jon Cuccui, Brendan W. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. 69: 85. Endpoint, kinetic, spectrum. Temperature Control: ambient from +4 to 45C. 96-well solid bottom, clear polystyrene microplates (Greiner cat. the assay. Fluorescence Microplate Readers. microplate . Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. 0 GB Brand Hewlett-Packard Hard driveThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. i3x Multi-Mode Microplate Reader. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. In simplest terms, a plate reader measures and. #L6876) • Guanidine hydrochloride (Sigma cat. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. It measures the optical density (OD) of samples in both 96- and 384-well. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Transfer of an assay to 96-well microplate technology increases throughput significantly. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 0 96 Well Plate. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Get. 2022 Harini Natarajan et al. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. , Sigma cat. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. 00 Add to cart; Molecular Devices EMax Microplate Reader $ 1,295. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. The Multiskan Sky microplate reader is an absorbance plate reader. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. Molecular Devices VersaMax Microplate Reader Specifications. Place the microplate in the drawer. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. 5°C at 37°C. best-in-class data . We would like to show you a description here but the site won’t allow us. Multimode Readers Comparison Chart. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Open the catalog to page 5. Bioz Stars score: 86/100, based on 1 PubMed citations. explore more. Unparalleled performance on a personalized platform. Running both standard spectrophotometer and microplate. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. #655075 and #781075) SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax Injector Cartridge *Figure 1. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. High. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesa solid, white, flat-bottom 96-well plate. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 6. 3. Step 1: Turn on your microplate reader. Microplate readers use a vertical light path so the distance of the. Triplicates of each BSA concentration were made for this assay plate. 0 OD Photometric resolution: 0. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. iD5. 0. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. The VersaMax and SpectraMax 190 read 96-well microplates. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Endpoint, kinetic, spectrum and area-well scanning Click here. for ValitaTiter Assays. Filters: Excitation and emission slides for 4 filters each. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. 2 OD at 200 nm; this decreased to 0. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. Molecular Devices i3x. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Wide range of applications The VersaMax reader covers. Industry standard, 96-well plates are. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Fluorescence Microplate Reader Comparison Chart. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . 2. absorbance, fluorescence, and luminescence with the added functionality of. High tolerance for contaminants. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. 63. This richly featured reader gives you the speed, convenience, and economy of monochromator-based wavelength selection for visible absorbance measurements between 340 nm and 850. A single-mode plate reader is a good starting point. or ValitaTiter Plus (Molecular Devices cat. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. explore more. . 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. No additional manuals are available. For more sample throughput, the system can well. Compact and tunable absorbance microplate readers DATA SHEET. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. fluorescence microplate reader from Molecular Devices. U-bottom: 30-40 µl. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Published Dec 16, 2020 | Updated Dec 16, 2020. Expt#3 . Wavelength Selection. Vrla used the device to quantify the concentration of a small molecule produced by. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. Cells were loaded with 2 µM Fluo-4 for one hour. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. the iD5 reader’s filter system. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. No accessories are needed and SoftMax® Pro Software. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Compare specifications and request a quote from leading suppliers. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. Efficient cell washing with minimal cell loss and optimal assay performance. The system provides automation for up to 50 microplates for easy walkaway automation. Microplate luminescent reader assay for ERK activity. 384 well polystyrene microplates have plate dimensions of 127. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. #I2511)Introduction. SpectraMax Microplate Readers. 54: 1. Figure 2. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. #G7294). 020 fmol 0. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Plates can put in the drawer both in landscape and portrait positions. 1 sec. Seller assumes all responsibility for this listing. 1536-well: 0. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. Microplate Capacity Standard 96-well microplates Incubation Temperature control up to 50ºC Temperature uniformity ± 0. 5 mm. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. 96- or 384-well microplate compatibility. Infinite 200 Pro Microplate Reader. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. Compact design Minimum volume and. Wavelength Range 200 to 999 nm. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. The Molecular Devices Versamax Microplate Reader INCLUDED:. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. 9. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. With various on-board features and capabilities, the ThermoMax is. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality.